MAT2A monoclonal antibody, clone AT3A2
产品名称： MAT2A monoclonal antibody, clone AT3A2
英文名称： MAT2A monoclonal antibody, clone AT3A2
产品编号： MAB3213
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant MAT2A.
- Immunogen:
- Recombinant protein corresponding to full length human MAT2A.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Leptin's mitogenic effect in human liver cancer cells requires induction of both methionine adenosyltransferase 2A and 2beta.
Ramani K, Yang H, Xia M, Ara AI, Mato JM, Lu SC.Hepatology. 2008 Feb;47(2):521-31.
- 2.
- Role of methionine adenosyltransferase 2A and S-adenosylmethionine in mitogen-induced growth of human colon cancer cells.
Chen H, Xia M, Lin M, Yang H, Kuhlenkamp J, Li T, Sodir NM, Chen YH, Josef-Lenz H, Laird PW, Clarke S, Mato JM, Lu SC.Gastroenterology. 2007 Jul;133(1):207-18. Epub 2007 Apr 11.
- Applications
- Western Blot (Cell lysate)
- Cell lysates of HepG2 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with MAT2A monoclonal antibody, clone AT3A2 (Cat # MAB3213 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Paraffin embedded sections of human colon cancer tissue were incubated with MAT2A monoclonal antibody, clone AT3A2 (Cat # MAB3213 ; 1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine (DAB).