FOXA1 monoclonal antibody (M01), clone 2D7
产品名称： FOXA1 monoclonal antibody (M01), clone 2D7
英文名称： FOXA1 monoclonal antibody (M01), clone 2D7
产品编号： H00003169-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant FOXA1.
- Immunogen:
- FOXA1 (NP_004487, 367 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- LASVPASHPAHGLAPHESQLHLKGDPHYSFNHPFSINNLMSSSEQQHKLDFKAYEQALQYSPYGSTLPASLPLGSASVTTRSPIEPSALEPAYYQGVYSRPVLNTS
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.4 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Association of GATA3, P53, Ki67 status and vascular peritumoral invasion are strongly prognostic in luminal breast cancer.
Jacquemier J, Charafe-Jauffret E, Monville F, Esterni B, Extra JM, Houvenaeghel G, Xerri L, Bertucci F, Birnbaum D.Breast Cancer Res. 2009;11(2):R23. Epub 2009 Apr 30.
- 2.
- Estrogen induces repression of the breast cancer and salivary gland expression gene in an estrogen receptor alpha-dependent manner.
Bretschneider N, Brand H, Miller N, Lowery AJ, Kerin MJ, Gannon F, Denger S.Cancer Res. 2008 Jan 1;68(1):106-14.
- Applications
- Western Blot (Cell lysate)
- FOXA1 monoclonal antibody (M01), clone 2D7. Western Blot analysis of FOXA1 expression in HepG2 ( Cat # L019V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody (M01), clone 2D7.
Lane 1: FOXA1 transfected lysate(49.1 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi ( Cat # H00003169-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA1 monoclonal antibody (M01), clone 2D7 (Cat # H00003169-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 3169
- GeneBank Accession#:
- NM_004496
- Protein Accession#:
- NP_004487
- Gene Name:
- FOXA1
- Gene Alias:
- HNF3A,MGC33105,TCF3A
- Gene Description:
- forkhead box A1
- Omim ID:
- 602294
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq
- Other Designations:
- hepatocyte nuclear factor 3, alpha
- Related Disease