IRAK4 (phospho T345) monoclonal antibody, clone A8A8
产品名称： IRAK4 (phospho T345) monoclonal antibody, clone A8A8
英文名称： IRAK4 (phospho T345) monoclonal antibody, clone A8A8
产品编号： MAB2538
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic phosphopeptide of IRAK4.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding T345 of human IRAK4.
- Sequence:
- VMTSR
- Host:
- Mouse
- Reactivity:
- Bovine, Dog, Human, Mouse
- Specificity:
- This antibody recognizes IRAK4 (phospho T345) with a phosphorylated site at Threonine 345. It does not cross-react with non-phosphospecific peptide.
- Form:
- Liquid
- Isotype:
- IgG1, kappa
- Storage Buffer:
- In PBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Augmentation of therapeutic responses in melanoma by inhibition of IRAK-1,-4.
Srivastava R, Geng D, Liu Y, Zheng L, Li Z, Joseph MA, McKenna C, Bansal N, Ochoa AC, Davila E.Cancer Res. 2012 Oct 4. [Epub ahead of print]
- 2.
- An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Lehmann SM, Kruger C, Park B, Derkow K, Rosenberger K, Baumgart J, Trimbuch T, Eom G, Hinz M, Kaul D, Habbel P, Kalin R, Franzoni E, Rybak A, Nguyen D, Veh R, Ninnemann O, Peters O, Nitsch R, Heppner FL, Golenbock D, Schott E, Ploegh HL, Wulczyn FG, Lehnardt S.Nat Neurosci. 2012 May 20. doi: 10.1038/nn.3113.
- 3.
- IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.
Li S, Strelow A, Fontana EJ, Wesche H.Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5567-72.
- Applications
- Western Blot (Cell lysate)
- The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1).
Lane 1 was pre-incubated with Phosphospecific peptide.
Lane 2 was pre-incubate with non-phospho specific peptide.
An immunoreactive band is observed around ~51 KDa.
- Entrez GeneID:
- 51135
- Gene Name:
- IRAK4
- Gene Alias:
- IPD1,NY-REN-64,REN64
- Gene Description:
- interleukin-1 receptor-associated kinase 4
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
- Other Designations:
- IRAK-4 mutated form 1,interleukin-1 receptor associated kinase 4
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